Collaborative Bioassay for Chick Edema Factor
نویسندگان
چکیده
منابع مشابه
Etiology of chick edema disease.
Chick edema disease first came to the attention of the Food and Drug Administration in December, 1957, when it was learned that millions of broilers died in the eastern and midwestern parts of the United States. Several groups in industry and government quickly determined that the disease was due to toxic components in certain feed fats (1-3), and that toxicity was associated with the unsaponif...
متن کاملDevelopment of an edema factor-mediated cAMP-induction bioassay for detecting antibody-mediated neutralization of anthrax protective antigen.
Intoxication of mammalian cells by Bacillus anthracis requires the coordinate activity of three distinct bacterial proteins: protective antigen (PA), edema factor (EF), and lethal factor (LF). Among these proteins, PA has become the major focus of work on monoclonal antibodies and vaccines designed to treat or prevent anthrax infection since neither EF nor LF is capable of inducing cellular tox...
متن کاملShort method for the detection of chick edema factor in fats, oils and fatty acids by electron capture gas chromatography.
The electron capture gas liquid chromatography test method for chick edema factor has been modified by replacing the alumina column s t ep with an additional sulfuric acid cleanup and a caustic wash which permits a reduction in sample cleanup time. Samples are subjected to double preliminary sulfuric acid cleanup and caustic wash and extracted each time with t r imethyl pentane. The final extra...
متن کاملA broiler chick bioassay for measuring the feeding value of wheat and barley in complete diets.
Energy is an important component of poultry feed and is derived principally from cereal grains. Unfortunately, all of the chemical energy is not available to the bird, and biological assays must be used to determine the digestible energy value of a cereal grain. The bioassay described uses four pens of six male broiler chicks, complete diets containing 80% of a test cereal grain (with or withou...
متن کاملCollaborative Study for the Establishment of a Rat Bioassay for Inactivated Poliomyelitis Vaccine
For many years a test in chicks or guinea pigs has been specified in the Ph. Eur.(2) as the assay for IPV (Poliomyelitis vaccine (inactivated), 1999:0214). However, a WHO collaborative study of immunogenicity assays of IPV found that these tests are poorly standardised (Wood and Heath 1995). An alternative test in rats (van Steenis et al. 1981, Bevilacqua et al. 1996) was found to be less varia...
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ژورنال
عنوان ژورنال: Journal of AOAC INTERNATIONAL
سال: 1961
ISSN: 0095-9111
DOI: 10.1093/jaoac/44.3.449a